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Half-life of MCC variant peptides from soluble IEk. (A) Fluorescein-labeled peptides and soluble IEk complexes were incubated for various times at 37°C with excess unlabeled peptide then analyzed by HPSEC. The peak height of the bound fluorescein peptide-MHC complexes was determined. The data are graphed as (natural log of Bound at time x/Bound at time 0) versus time. (B) Half-life values for each peptide as derived from the slope of the graph, t1/2 = natural log 2/slope of curve.
