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. 2002 Mar 26;99(7):4544–4549. doi: 10.1073/pnas.082647499

Figure 2.

Figure 2

The loss of IL-2 production by J.SL1 cells can be reconstituted by the expression of exogenous Shc. (A) Total cell lysates from Jurkat, J.SL1, and Shc-reconstituted J.MAK14 cells were analyzed by Western blot by using anti-Shc antibody. (B) The culture supernatants from unstimulated (lanes 1, 3, and 5) and anti-TCR stimulated (lanes 2, 4, and 6) Jurkat, J.SL1, and J.MAK14 cells were assayed for IL-2 secretion. (C) Total RNA samples from unstimulated (lanes 1, 2, and 3) and stimulated (lanes 4, 5, and 6) Jurkat, J.SL1, and J.MAK14 cells were analyzed by Northern blot with human IL-2 cDNA (Upper). The same filter was rehybridized with glyceraldehyde-3-phosphate dehydrogenase (G3PDH) cDNA (Lower) to show that equivalent amounts of RNA were loaded.