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HLTF silencing in colon cancer cell lines. (A) HLTF RNA expression. Shown is RT-PCR assay for HLTF expression in colon cancer cell lines. (B) HLTF expression reactivation. Shown is RT-PCR assay for HLTF expression in colon cancer cell lines treated (+) or untreated (−) with 5-azaC. Cell lines V429 and V503 are controls with constitutive HLTF expression. 5-AzaC treatment reactivates HLTF expression in cell lines RCA, V457, SW480, V5, V6, and V432.
