Figure 2.

HLTF promoter methylation. (A) Diagram of the putative HLTF promoter. CpG sites are shown with circles. Shaded circles represent CpG sites that are tested in MS-PCR assays. Hatched circles represent CpG sites that overlap HpaII restriction sites. (B) MS-PCR assay of the putative HLTF promoter. Shown are the results of MS-PCR assay of the putative HLTF promoter by using primers specific for amplification of either methylated (M) or unmethylated (U) templates. (C) HLTF MS-PCR of matched cell lines and tissues. Shown are the results of HLTF MS-PCR assay of colon cancer cell lines (C), matched antecedent tumor tissue (T), or matched normal colon mucosa (N).