Figure 5.

HLTF colony suppressor activity. Shown are the number of G418-resistant colonies arising from transfection with an HLTF expression vector (HLTF) or a control empty expression vector (pcDNA) in HLTF unmethylated and expressing FET, V364, and V429 cells (A) as compared with HLTF-methylated and -deficient V457, V8–2, and RCA cells (B). (C) Anti-V5 Western blot assay of V5 epitope-tagged HLTF introduced by transient transfection into HLTF-methylated versus unmethylated cells. Control cells were transfected with an empty expression vector (pcDNA3.1).