Figure 5.

(a) Microscopic image (×10) of CFU colonies counting on day 14 of MethoCult culture of the pre-expansion MNCs. (b)- Microscopic image (×10)of CFU colonies counting on day 14 of MethoCult culture of the post-expansion MNCs. To count the colonies easier under microscope tow blue perpendicular marker lines were drawn on the back bottom of the MethoCult culture plates. (c) (40×) (By the high power inverted microscope), The CFU colonies in the Methocult medium culture at day 14 of culture of the post-expansion MNCs. The low power used first to determine the CFU count, then the high power used to detect the morphology. The colonies were mostly CFU-GM.