Table 1.

Viable MNCs count assessment and viability assessment by Trypan blue

Sample number	Day 0	Day 3	Day 7	Day 10	Day 13
	Number of viable selected MNCs seeded	Viable count (% viability)	Viable count (% viability)	Viable count (% viability)	Viable count (% viability)
1	50,000	40,600 (28%)	22,440 (20 %)	89,760 (33 %)	1,600,000 (72 %)
2	50,000	48,865 (29 %)	37,455 (23 %)	432,300 (52 %)	1,050,000 (66 %)
3	50,000	396,900 (63 %)	306,900 (65 %)	254,430 (68 %)	905,000 (62 %)
4	50,000	63,440 (52 %)	37,455 (45 %)	584,100 (70 %)	1,200,000 (69 %)
5	50,000	854,700 (61 %)	3,668,500 (64 %)	8,400,000 (82 %)	8,750,000 (70 %)
6	50,000	158,550 (67 %)	1,023,000 (77 %)	1,395,000 (75 %)	3,950,000 (60 %)
7	50,000	4,935,000 (82 %)	4,488,000 (80 %)	3,232,500 (74 %)	3,320,000 (58 %)
8	50,000	1,414,000 (70 %)	1,298,000 (61 %)	5,407,500 (48 %)	12,000,000 (83 %)
9	50,000	285,600 (51 %)	1,848,000 (59 %)	3,570,000 (70 %)	7,350,000 (47 %)
10	50,000	254,100 (76 %)	6,765,000 (82 %)	3,840,000 (43 %)	15,400,000 (84 %)
11	50,000	2,681,000 (79 %)	671,000 (73 %)	6,090,000 (77 %)	8,390,000 (69 %)
12	50,000	889,000 (50 %)	2,843,500 (70 %)	9,825,000 (56 %)	19,300,000 (80 %)
13	50,000	1,015,000 (27 %)	2,117,500 (77 %)	4,417,500 (40 %)	6,940,000 (55 %)
14	50,000	1,792,000 (82 %)	8,690,000 (77 %)	12,750,000 (87 %)	12,000,000 (49 %)
15	50,000	222,250 (78 %)	1,149,500 (61 %)	1,635,000 (72 %)	6,890,000 (75 %)
16	50,000	1,809,500 (79 %)	8,085,000 (83 %)	3,232,500 (82 %)	3,720,000 (59 %)
17	50,000	602,000 (51 %)	1,721,500 (28 %)	2,250,000 (35 %)	4,400,000 (36 %)
18	50,000	6,650,000 (82 %)	2,046,000 (70 %)	12,975,000 (62 %)	7,300,000 (68 %)
19	50,000	714,000 (68 %)	9,185,000 (85 %)	9,150,000 (86 %)	6,350,000 (72 %)
20	50,000	714,000 (65 %)	448,800 (72 %)	9,450,000 (85 %)	6,210,000 (42 %)