Table 4.
Colony forming unit (CFU) assay (clonogenic assay): Average CFU count / 105 MNCs
| Sample | CFU count | Fold change | |
|---|---|---|---|
| Day 0 | Day13 | ||
| 1 | 667 | 1.16 | 576 |
| 2 | 1515 | 2.63 | 576 |
| 3 | 1054 | 1.83 | 576 |
| 4 | 1757 | 3.05 | 576 |
| 5 | 7272 | 14.12 | 515 |
| 6 | 6666 | 12.94 | 515 |
| 7 | 1394 | 2.71 | 515 |
| 8 | 2060 | 4.00 | 515 |
| 9 | 1212 | 2.35 | 515 |
| 10 | 4242 | 8.24 | 515 |
| 11 | 3636 | 2.67 | 1364 |
| 12 | 4848 | 3.55 | 1364 |
| 13 | 3030 | 2.22 | 1364 |
| 14 | 7272 | 5.33 | 1364 |
| 15 | 4242 | 3.11 | 1364 |
| 16 | 3333 | 2.44 | 1364 |
| 17 | 4545 | 3.33 | 1364 |
| 18 | 4696 | 3.44 | 1364 |
| 19 | 2181 | 1.60 | 1364 |
| 20 | 6060 | 4.44 | 1364 |
| Median (Min-Max) | 3484.5 (667-7272) | 3.08 (1.16-14.12) | 970 (515-1364) |
Average CFU count / 105 MNCs assessed preculture (day 0) & post culture (day 13) & calculation of fold change in the CFU counts by dividing day 13 on day o counts. It is important to mention that only three Methocult cultures were done at day 0, one Methocult culture for each CB bag used to separate the MNCs, that explain why the number of colonies at day 0 was the same for samples (1-4), (5-10) & (11-20).
Wilcoxon signed rank test was used and it shows statistically significant increase in the post culture CFU counts (p-value<0.001) in comparison with the preculture CFU counts.