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. 2025 Jul 14;207(8):e00159-25. doi: 10.1128/jb.00159-25

Fig 5.

Sequence alignment and structural model of TP0548 highlight extensive intra- and inter-clade mutations across Nichols and SS14 subclades. Variants cluster in extracellular loops and hatch domain, with mapped substitutions on β-barrel structure.

TP0548 is hypervariable with Nichols- and SS14-specific substitutions within the hatch and ECL2, respectively. (A) Amino acid residue differences between the 18 identified TP0548 proteoforms (1–18 in the leftmost squares). Reference sequences for Nichols and SS14 contain 25 inter-clade mutations (peach boxes). We identified 16 variant proteoforms within our cohort, eight in each clade, containing intra-clade substitutions (gray-blue boxes) at 15 amino acid positions. Most of the intra-clade differences are found in the hatch and in ECL2, for the Nichols-lineage and SS14-lineage strains, respectively. Non-conservative substitutions are colored in red. Residues are numbered according to their position in the Nichols reference sequence. (B) Substitutions were mapped onto the AlphaFold3 model for the Nichols reference.