TABLE 1.
Strain | Reportera | β-Gal activityb | LacZ mRNA abundancec | Corrected β-Gald | % PRF correctede |
Wild type | Control | 14.24 (± 1.14) | 1.88 | 7.57 (± 0.60) | |
Wild type | L-A | 0.28 (± 0.04) | 0.88 | 0.31 (± 0.05) | 4.14 (± 0.74) |
upf1Δ | Control | 15.88 (± 0.94) | 2.54 | 6.25 (± 0.37) | |
upf1Δ | L-A | 1.14 (± 0.08) | 2.80 | 0.41 (± 0.05) | 6.52 (± 0.90) |
alacZ monocistronic reporter plasmids. Control: lacZ in-frame with AUG. L-A: contains L-A derived -1 PRF signal, lacZ in -1 frame with AUG.
bβ-Galacosidase assays were performed in triplicate on at least three separate occasions.
cAverage LacZ mRNA abundance calculated as the ratio of LacZ to 5S rRNA signal from two independent experiments.
dβ-Galacosidase activities were divided by the average LacZ mRNA abundance in each strain.
e% PRF = (corrected β-Gal L-A/corrected β-Gal control) ·100%.