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Time course of 3OC8HSL inactivation by protein extracts and the AHL-lactonase encoded by attM. Symbols: ■, M103 total protein; □, denatured M103 total protein; ▴, A6 total protein; ▵, denatured A6 total protein. ●, purified AHL-lactonase encoded by attM (1 μM final concentration in 1 × PBS buffer, pH7.4); ○, denatured AHL-lactonase.
