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. 2002 Mar 19;99(7):4638–4643. doi: 10.1073/pnas.022056699

Figure 3.

Figure 3

Demonstration that attM encodes an AHL-lactonase. (A) HPLC analysis of the AHL-lactonase digestion product of 3OC8HSL. (Upper) HPLC profile of 3OC8HSL (retention time = 8.3 min), the minor peak at 7.7 min was the sodium salt of 3OC8HSL based on MS analysis (data not shown). (Lower) Profile shows that 3OC8HSL digestion by AHL-lactonase results in one product with a retention time of 5.3 min. (B) ESI-MS analysis of the hydrolysis product of 3OC8HSL. The fraction at 5.3 min from HPLC was collected and analyzed by ESI-MS.