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. 2002 Mar 19;99(7):4638–4643. doi: 10.1073/pnas.022056699

Figure 4.

Figure 4

RT-PCR analysis of the attK-attL-attM operon. (A) Map positions of attJ, attK, attL, and attM genes and the primers for RT-PCR analysis. (B) RT-PCR analysis of expression profiles of the attK-attL-attM operon in strain A6 and M103. The RT-PCRs were performed by using three primer-pair combinations as illustrated by short arrows in A. To each RT-PCR, a pair of PCR primers specific for amplification of a 1.2-kb 16S rDNA fragment were added as an internal control to indicate quality and relative quantity of cDNA samples. Symbols: M, 1-kb DNA marker; RNA, RT-PCR profile when RNA sample from either A6 or M103 was used as a template.