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. 2002 Mar 19;99(7):4638–4643. doi: 10.1073/pnas.022056699

Figure 5.

Figure 5

AttJ specifically binds to the promoter of the attK-attL-attM operon. (A) The promoter sequence of attK-attL-attM operon. The start (791) and end (1003) sites of this fragment correspond to the designations in Fig. 4A. The arrow indicates the direction of transcription, and the star indicates the translation start site of attK. (B) DNA gel retardation analysis. Lane 1 is the negative control of labeled probe without AttJ protein. Purified AttJ was incubated at decreasing concentrations (100, 50, and 10 nM) with the labeled attK-attL-attM promoter probe (lanes 2–4); labeled probe plus a 100-fold excess of unlabeled probe (lanes 5–7); labeled probe plus 100-fold excess of unlabeled 213-bp lac promoter fragment (lanes 8–10).