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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: DNA Repair (Amst). 2005 Aug 15;4(9):1038–1046. doi: 10.1016/j.dnarep.2005.05.004

Fig. 2. Analysis of GCV resistant clones.

Fig. 2

HindIII digested genomic DNA from individual clones hybridized to a neo gene specific (left panel) or a chromosome 7 (TV 7) specific probe (right panel). In most cases, the two hybridization signals are of the same size, indicating that repair of the DSB was accompanied by an interstitial deletion. In clone 5-4, the deletion extended beyond the neoR gene (verified by inverse PCR sequence analysis). In clone 5-22, the chromosome seven specific probe shows two signals of even intensity, neither of whose size corresponds to that of the neo specific signal (arrows). The endogenous chromosome seven signal is seen at 2.3 kb in all lanes. Size standards are in kb.