Fig. 3. Diagram of genetic changes around the DSB site.
Representative examples of different types of rearrangements are shown. Numbers on vector DNA represent nucleotide position; sequences downstream of neor gene are not shown. The staggered I-SceI cleavage site is underlined. Clone 5-115, 5-121, 22-8, 22-29 and 227/2 contain an insertion that matches distant regions of the human genome; clone 5-115 also contain an insertion of 14 bp segment of low complexity. Clone 5-133, 6-5 and 6-28 sustained more complex rearrangements involving a primary deletion and duplication leading to the formation of direct repeats. Clone 5-155 contains two insertions from adjacent chromosome 7 sequences; clone 6-18a contains an inversion of a segment of the HSV-tk gene. Δ refers to nucleotide deletion from EF1α or HSV-tk; μH indicates microhomology at each junction; del, ins, inv and dupl refer to deletions, insertions, inversions or duplications, respectively. Open rectangles represent duplications, and letters (abc, def, and ghi) indicate the orientation of repeats. Figure is not drawn to scale.