Table 1.
Features of non vector derived DNA insertion events.
| clone | match for inserted DNA | description of inserted DNA | features of breakpoint junction |
|---|---|---|---|
| 22-7/2 | 6q22.1 and Xq13.1 | mRNA (gb BU660518.1) (nuc. 114,938,877-114,939,020) and histone deacetylase 8 (HDAC8) gene (nuc. 69,839,437-69,839,474 | del. of 7 and 12 bp μH of 1, 1 and 0 bp |
| 22-8 | 4q35.2 | D4Z4 repeat, ORF inside (nuc. 191,697,731-191,697,966) | del. of 20 and 45 bp μH of 0 and 2 bp |
| 22-29 | 21q21.1- q21.2 | L1 LINE and flanking unique sequence (nuc. 22,512,921-22,513,550) | staggered cut of I-SceI site is filled in, del. of 1 and 0 bp, μH of 3 and 0 bp |
| 5-115 | 4q31.23 | intronic sequence within the human mineralocorticoid receptor gene (nuc. 149631931-149632077) | extra 14 bp CT-rich sequence added, forms direct repeat with breakpoint junction, del. of 6 and 807 bp, μH of 0 bp |
| 5-121 | 6p21.1 | 3′ UTR of translocating chain-associating membrane protein 2 (TRAM2) gene (nuc. 52364732-52364778) | del. of 0 and 432 bp μH of 0 bp |
| 29-6 | 10q26.13 | intronic region of arginyl-tRNA-protein transferase (ATE1) gene (nuc. 123208457 123208689) | staggered cut of I-SceI site is filled in, μH of 4 bp |
| 29-45 | 12p11.21 and 12p13 | intronic region of DDX12, a duplicated putative helicase gene (nuc. 31140689-31140834, 9469691- 9469836 and 9349729-9349874) | del. of 17 and 0 bp μH of 1 and 1 bp |
| 29-1A | 4q35.2 and 10p13 | two distinct segments of non coding DNA (nuc. 188535445 188535618 and 16305232-16305415) | del. of 18 and 1 bp, μH of 1, 1 and 0 bp |