Skip to main content
. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: J Biol Chem. 2005 Apr 4;280(22):21091–21098. doi: 10.1074/jbc.M500754200

Fig 1. Interaction of SRC-1 with NF-kB.

Fig 1

(A) ChIP assay for SRC-1/NF-kB association. The signals for p65, p50 and Pol II indicate that NF-kB was activated and mRNA synthesis was initiated. Actin signal is a control of DNA input. (B) Cotransfection of SRC-1 with IkBα reporter. The IkBα reporter was activated by cotransfection of p65 and p50 expression vectors, which were used at 0.1 μg/point for each plasmid DNA. (C) Immunoblotting of IkBα protein in HEK293 cells transfected by SRC-1. SRC-1 expression is confirmed in the transfected cells. Actin protein is a control for protein loading. (D) Cotransfection of SRC-1 with NF-kB luciferase reporter. The same condition was used as described in the legend for panel 2 except the reporter DNA.