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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: J Biol Chem. 2005 Apr 4;280(22):21091–21098. doi: 10.1074/jbc.M500754200

Fig 2. Substitution of SRC-2 by SRC-1.

Fig 2

(A) ChIP assay for SRC-2/NF-kB association. This result was obtained in the same condition as for ChIP assay of SRC-1. The signals for p65, p50 and Pol II is presented in Fig. 1A. (B) Nuclear exclusion of SRC-2. The cytoplasmic and nuclear proteins were extracted from HEK293 cells after TNF-treatment. SRC-2 protein was quantified in the extracts by immunoblotting. Actin and SP1 protein signals are used as controls for protein loading of the cytoplasmic and nuclear extracts, respectively. (C) Cotransfection of SRC-2 with IkBα reporter. The IkBα reporter was activated by cotransfection of p65 expression vectors at 0.1 μg/point. (D) Immunoblotting of IkBα protein in HEK293 cells transfected. SRC-2 protein level is confirmed in cells transfected for overexpression or knockdown.