Skip to main content
. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: J Biol Chem. 2005 Apr 4;280(22):21091–21098. doi: 10.1074/jbc.M500754200

Fig 3. Oscillation of SRC-3.

Fig 3

(A) ChIP assay for SRC-3/NF-kB association. This result was obtained in the same condition as for ChIP assay of SRC-1. The signals for p65 and p50 demonstrates activation of NF-kB by TNF-α. (B) Cotransfection of SRC-3 with IkBα reporter. The IkBα reporter was activated by cotransfection of p65 expression vector at 0.1 μg/point. The SRC-3 DNA (μg) is indicated. (C) Knockdown of SRC-3 by vector-based RNAi expression. The DNA (μg) of SRC-3 RNAi vector is indicated. (D) Immunoblotting of IkBα protein in HEK293 cells transfected. SRC-3 protein level is confirmed in cells transfected for knockdown.