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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: J Biol Chem. 2005 Apr 4;280(22):21091–21098. doi: 10.1074/jbc.M500754200

Fig 4. Inhibition of NF-kB by HDAC1.

Fig 4

(A) ChIP assay for HDAC1/NF-kB association. This result was obtained in the same condition as for ChIP assay of SRC-1. (B) Cotransfection of HDAC1 with IkBα reporter. The IkBα reporter was activated by cotransfection of p65 expression vector at 0.1 μg/point. The HDAC1 DNA (μg) is indicated. (C) Knockdown of HDAC1 by vector-based RNAi expression. The DNA (μg) of HDAC1 RNAi (1RNAi) vector is indicated. (D) Immunoblotting of IkBα protein in HEK293 cells transfected. HDAC1 protein level is confirmed in cells transfected for overexpression knockdown.