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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: J Biol Chem. 2005 Apr 4;280(22):21091–21098. doi: 10.1074/jbc.M500754200

Fig 5. HDAC2/NF-kB interaction.

Fig 5

(A) ChIP assay for HDAC2/NF-kB association. This result was obtained in the same condition as for ChIP assay of SRC-1. (B) Cotransfection of HDAC2 with IkBα reporter. The IkBα reporter was activated by cotransfection of p65 expression vector, which was used at 0.1 μg/point. The HDAC2 DNA (μg) is indicated. (C) Knockdown of HDAC2 by vector-based RNAi expression. The DNA (μg) of HDAC2 RNAi vector is indicated. 2RNAi is abbreviation of HDAC2 RNAi. (D) Immunoblotting of IkBα protein in HEK293 cells transfected. The change in HDAC2 protein is confirmed in cells transfected for overexpression knockdown.