Figure 8.

Characterization of lungs from Cd73 ^−/− mice. Wild-type (A and D) or Cd73 ^−/− (B and C) mice were subjected to normoxia (A and B) or hypoxia (C and D). Whole lungs were fixed with 10% formalin at total lung capacity, sectioned, and stained with hematoxylin and eosin. (A) A representative image of a wild-type control animal (magnification, 100). (C) Perivascular interstitial edema (line arrows) and epithelial disruption (block arrow) in Cd73 ^−/− hypoxic mice (magnification, 100). (D) Subtle perivascular interstitial edema (line arrow) associated with wild-type hypoxic mice (magnification, 100). (E) Assessment of lung water content in normoxia (black bars) and hypoxia (gray bars) in the presence and absence of NECA administration (0.1 mg/mg i.p. plus 0.1 mg/kg s.c.). Data are expressed as mean ± SD mg H₂O/mg dry tissue and are pooled from three to four animals per condition where, in comparisons between hypoxia and normoxia, * indicates P < 0.025 and, in comparisons between NECA and PBS, # indicates P < 0.025.