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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: Genomics. 2002 Jul;80(1):54–61. doi: 10.1006/geno.2002.6800

FIG. 6.

FIG. 6

Mouse INSM1 interacts with CAP in vitro. (A) Expressed and purified GST (lane 1) and GST–CAP (lane 2) fusion proteins were electrophoresed on a 12% SDS-PAGE and stained by Coomassie blue. (B) The GST–CAP fusion protein was immobilized on glutathione Sepharose beads and then incubated with 35S-labeled INSM1. After extensive washing, bound [35S]INSM1 was eluted and separated on a 12% SDS-PAGE. Lane 3 shows that the 58-kDa 35S-labeled INSM1 protein interacted with and was pulled down by GST–CAP, but not by GST alone (lane 2). The first lane, loaded with 35S-labeled INSM1 protein, served as a size control.