Table 1.

Cryo-EM data collection, refinement and validation statistics.

	#1 FOXP3ΔN_TTG 24 (EMDB-46499) (PDB: 9D2L )	#2 FOXP3ΔN_TTT TG15_3DNA (EMDB-46480) (PDB:9D22)	#3 FOXP3ΔN_TTT TG15_2DNA_1 (EMDB-46493) (PDB:9D2E)	#4 FOXP3ΔN_TTTT G15_2DNA_2 (EMDB-46498) (PDB: 9D2J)
Data collection and processing
Magnification	105000	105000	105000	105000
Voltage (kV)	300	300	300	300
Electron exposure (e–/Å2)	60	60	60	60
Defocus range (μm)	−0.8 to −2.2	−0.8 to −2.2	−0.8 to −2.2	−0.8 to −2.2
Pixel size (Å)	0.827	0.827	0.827	0.827
Symmetry imposed	C2	C1	C1	C1
Initial particle images (no.)	2535992	1327974	1327974	1327974
Final particle images (no.)	1340131	630564	195660	259858
Map resolution (Å)	3.0	2.6	2.9	2.8
FSC threshold	0.143	0.143	0.143	0.143
Refinement
Initial model used (PDB code)	7TDX	7TDX	7TDX	7TDX
Model resolution (Å)	3.1	2.9	3.1	3.1
FSC threshold	0.5	0.5	0.5	0.5
Map sharpening B factor (Å2)	−168.5	−75.0	−54.9	−75.3
Model composition
Non-hydrogen atoms	24688	13390	9580	9304
Protein residues	2471	968	705	695
Ligands	386	264	186	186
R.m.s. deviations
Bond lengths (Å)	0.004	0.003	0.005	0.004
Bond angles (°)	0.596	0.562	0.635	0.595
Validation
Clashscore	8.64	6.58	12.73	8.79
Poor rotamers (%)	3.41	3.50	4.31	4.52
Ramachandran plot
Favored (%)	92.92	93.87	94.19	91.90
Allowed (%)	7.08	6.13	5.81	8.10
Disallowed (%)	0	0	0	0