| Antibodies |
| Mouse Anti-Iba1 |
Santa Cruz Biotechnology |
Sc-32725 |
| Rabbit Anti-Phox2b |
Cell Signaling Technology |
83811S |
| Rat Anti-CD45 |
R&D Systems |
MAB114-SP |
| Rat Anti-CD80 |
ThermoFisher Scientific |
12-0801-82 |
| Rat Anti-CD11b |
BioLegend |
101241 |
| Sheep Anti-cFos |
Sigma Aldrich |
CBL440 |
| Donkey anti-rabbit IgG |
Abcam |
Ab98799 |
| Donkey anti-mouse IgG |
Abcam |
Ab97085 |
| Rabbit anti-OTC |
Abcam |
Ab203859 |
| Mouse anti-β-actin |
Sigma-Aldrich |
A5441 |
| Rabbit anti-HNF4α |
Abcam |
ab181604 |
| Rabbit anti-vinculin |
Cell signaling |
13901S |
|
|
|
| Bacterial and virus strains |
| AAV8-shLUCIFERASE |
Weizmann institute of science |
N/A |
| AAV8-shHNF4α |
Weizmann institute of science |
N/A |
| Chemicals, peptides, and recombinant proteins |
| DMEM |
Life Technologies |
11995-065 |
| FBS |
Life Technologies |
16000-044 |
| BEGM BulletKit |
Lonza |
CC-3171 & CC-4175 |
| Acetylcholine Chloride |
Sigma Aldrich |
A2661 |
| Matrigel |
Corning |
356234 |
| Cisplatin |
Sigma Aldrich |
232120-50mg |
| Methoxyamine hydrochloride |
ThermoFisher |
A19188.06 |
| N,O-bis (trimethylsilyl) trifluoroacetamide |
Sigma Aldrich |
15222 |
| Wheat Germ Agglutinin, AlexaFluor™ 488 Conjugate |
ThermoFisher |
W11261 |
| RIPA lysis buffer |
Sigma Aldrich |
R0278 |
| Protease Inhibitor Cocktail |
Sigma Aldrich |
P8340 |
| Phosphatase Inhibitor Cocktail |
Sigma Aldrich |
P5726 |
| ECL |
ThermoFisher |
32106 |
| Anamorelin |
Santa Cruz Biotechnology |
SC-480157 |
| CCL2 |
PeproTech |
250-10 |
| Bindarit |
TargetMol |
T6413 |
| Hematoxylin Stain |
Leica Biosystems |
3801560 |
| Eosin Y |
Sigma-Aldrich |
HT110216 |
| Hexamethonium |
Sigma-Aldrich |
H0879 |
| Atropine |
Sigma-Aldrich |
PHL84121 |
| Critical commercial assays |
| VETSCAN Liver Profile Cassette |
Abaxis |
500-0040 |
| Direct-zol RNA MiniPrep kit |
Zymo |
ZR-R2050 |
| Rneasy Mini Kit |
Qiagen |
74104 |
| qScript cDNA Synthesis Kit |
Quanta |
95749 |
| SYBR Green FastMix perfect CT |
Quantabio |
95073 |
| BCA Protein Assay Kit |
ThermoFisher |
23225 |
| Human CCL2 ELISA |
PeproTech |
BGK13500 |
| ProcartaPlexTM Human Immune Response Panel, 80plex |
Thermofisher Scientific |
EPX800-10080-901 |
| Mouse CCL2 ELISA |
R&D Systems |
MJE00B |
| Acetylcholine ELISA |
BIOMATIK |
EKE62518 |
| Experimental models: Cell lines |
| Mouse: KPC4508P pancreatic cancer |
Cancer Tools |
153474 |
| Mouse: LL/2 (LLC1) lung cancer |
ATCC |
CRL-1642 |
| Human: THLE-2 hepatocyte |
ATCC |
CRL-2706 |
| Experimental models: Organisms/strains |
| Mouse: C57Bl/6J |
Jackson Laboratory; Envigo |
000664; 00800124-2 |
| Oligonucleotides |
| See Table S2
|
|
|
| Software and algorithms |
| StimWare |
Triangle BioSystems International |
N/A |
| LabView SignalExpress |
National Instruments |
N/A |
| MATLAB |
MathWorks |
N/A |
| MassHunter |
Agilent Technologies |
N/A |
| FIJI |
Schindelin, et al.118
|
https://imagej.net/software/fiji/
|
| Muscle Morphometry Plug-In |
Anthony Sinadinos using Eclipse IDE |
https://drive.google.com/drive/folders/0B_bBI7SbDQhCR1MxNEVXSlhiekE?resourcekey=0-8wdIKyTc0OKlB7WN67JqIw
|
| ImageLab |
Bio-Rad |
Version 6.1 |
| JMP Pro 16 |
JMP Statistical Discovery |
N/A |
| Prism |
GraphPad |
Version 9.4.1 |
| Other |
| Stimulation Headstage |
Duke University |
N/A |
| AWG40 Hookup Wire |
Cooner Wire Company |
AS 631 |
| Loctite 405 adhesive |
Ellsworth |
135436 |
| Restraint Cone |
Fisher Scientific |
14-370-110 |
| AC Amplifier |
A-M Systems |
Model 1800 |
| BNC Adapter |
National Instruments |
BNC-2110 |
| Lithium-Heparin Tubes |
VetMarket |
5598006 |
| VETSCAN VS2 |
Abaxis |
VETSCAN VS2 |
| 3.2mm beads |
Next Advance |
ADV-SSB32 |
| CryoMill |
Retscht |
N/A |
| Nitrocellulose membrane |
TAMAR |
10401383 |
| Graphene Ink |
Sigma-Aldrich |
793663 |
| Silicone Adhesive |
DIGI-KEY |
1067-3M2477P-4-10-ND |
