Figure 3.

(A) Effect of antibiotics. Gentamicin (100 μg/ml) or rifampin (5 μg/ml) was added at indicated times after infection. At 30 h, HUVEC cultures were serum-starved overnight in the presence of antibiotics, and cell viability was determined (in quadruplicate). (B) Endothelial viability after exposure to conditioned medium. Tester endothelial cells were treated with bacterially conditioned media made in the presence (+) or absence (−) of HUVECs and then serum-starved for 18 h (in sextuplicate).