Table 2.
Measurement | Control | HG | Taxol | Urethane |
---|---|---|---|---|
Polar anaphase astral MT growth rate (μm/min) | 10 ± 2 | 8.2 ± 2 | na* | na* |
Equatorial anaphase astral MT growth rate (μm/min) | 8.3 ± 2 | 8.0 ± 2 | na* | na* |
Rate of anaphase chromosome segregation (μm/min) | 4.0 ± 0.5 | 4.0 ± 1 | 3.7 +/- 1 | 4.2 ± 0.5 |
Rate of furrow ingression (μm/min) | 15 ± 2 | 12 ± 1 | 10 ± 5 | na* |
Time from anaphase onset to furrow initiation (min) | 13 ± 0.5 | 9.5 ± 1 | na* | na* |
All measurements were made on time-lapse stacks of either GFP-EB1-microinjected or Hoechst-stained cells, acquired using either Leica or MetaMorph imaging software. Each measurement represents the mean value obtained from at least five independent measurements. ± represents the range of data.
na indicates either that we were unable to make accurate measurements of the dynamic parameter being assessed or that the parameter was not relevant under the condition being tested.