Figure 6.

Function of the N-terminal deletion Gpa2 proteins in vivo. (A) Schematic of N-terminal deletion Gpa2 variants and complementation results in gpa2 or gpr1 gpa2 mutant cells. N-terminal deletions were created in the wild-type GPA2 gene and introduced into gpa2 (MLY132α for invasive growth assay and MLY132a/α for pseudohyphal growth assay) or gpr1 gpa2 (MLY277a/α) mutant cells and ability to complement pseudohyphal and invasive growth defects was examined. Representative data are shown in B for pseudohyphal growth and in C for invasive growth. (D) Glucose-induced cAMP production in gpa2 (MLY132α) mutant cells expressing the N-terminal deletion Gpa2 derivatives. The values shown are the mean of two independent experiments, except the control, which is representative of cells carrying the empty vector (pTH19).