Table 2.
Plasmids
| Plasmid | Description | Source/Reference |
|---|---|---|
| pTH19 | PADH1URA3 2μ | This study |
| pTH26 | PADH1-GPB1 URA3 2μ (pTH19) | This study |
| pTH27 | PADH1-GPB2 URA3 2μ (pTH19) | This study |
| pTH47 | PADH1-GPA2 URA3 2μ (pTH19) | This study |
| pTH48 | PADH1-GPA2Q300L URA3 2μ (pTH19) | This study |
| pTH49 | PADH1-GPA2G299A URA3 2μ (pTH19) | This study |
| pTH62 | PADH1-GPA2G2A URA3 2μ (pTH19) | This study |
| pTH65 | PADH1-GPA21-30 aa::GFP URA3 2μ (pTH19) | This study |
| pTH68 | PADH1-GPA2C4A URA3 2μ (pTH19) | This study |
| pTH69 | PADH1-GPA2S6Y URA3 2μ (pTH19) | This study |
| pTH71 | PADH1-GPA21-10 aa::GFP URA3 2μ (pTH19) | This study |
| pTH73 | PADH1-GFP URA3 2μ (pTH19) | This study |
| pTH75 | PADH1-GFP-GPB2 URA3 2μ (pTH19) | This study |
| pTH80 | PADH1-GPA21-10::GFP::GPA24-449 URA3 2μ (pTH19) | This study |
| pTH81 | PADH1-GPA21-20 aa::GFP-FLAG URA3 2μ (pTH19) | This study |
| pTH84 | PADH1-GFP-GPB2 LEU2 2μ (pTH171) | This study |
| pTH91 | PADH1-GPA21-20 aa G2A::GFP-FLAG URA3 2μ (pTH19) | This study |
| pTH92 | PADH1-GPA21-20 aa C4A::GFP-FLAG URA3 2μ (pTH19) | This study |
| pTH93 | PADH1-GPA21-20 aa S6Y::GFP-FLAG URA3 2μ (pTH19) | This study |
| pTH100 | PADH1- GFP-FLAG URA3 2μ (pTH19) | This study |
| pTH103 | PADH1-GPA11-20 aa::GFP-FLAG URA3 2μ (pTH19) | This study |
| pTH106 | PADH1-GFP-GPB1 URA3 2μ (pTH19) | This study |
| pTH114 | PADH1-GPB2 LEU2 2μ (pTH171) | This study |
| pTH127 | PADH1-GPA11-10-GPA2Δ1-100 URA3 2μ (pTH19) | This study |
| pTH128 | PADH1-GPA11-10-GPA2Δ1-100 G299A URA3 2μ (pTH19) | This study |
| pTH130 | PADH1-GPA2Δα (51-57) G299A URA3 2μ (pTH19) | This study |
| pTH133 | PADH1-GPA2Δα (51-57) URA3 2μ (pTH19) | This study |
| pTH134 | PADH1-GPA11-10-GPA2Δ1-29 G299A URA3 2μ (pTH19) | This study |
| pTH136 | PADH1-GPA2Δ16-84 G299A URA3 2μ (pTH19) | This study |
| pTH144 | PADH1-GPA11-10-GPA2Δ1-14 G299A URA3 2μ (pTH19) | This study |
| pTH145 | PADH1-GPA2Δ46-84 G299A URA3 2μ (pTH19) | This study |
| pTH149 | PADH1-GPA2G2A-NLS URA3 2μ (pTH19) | This study |
| pTH155 | PADH1-GPA2Δ46-100 URA3 2μ (pTH19) | This study |
| pTH157 | PADH1-GPA11-10-GPA2Δ1-29 URA3 2μ (pTH19) | This study |
| pTH158 | PADH1-GPA2Δ46-84 URA3 2μ (pTH19) | This study |
| pTH159 | PADH1-GPA2Δ31-84 G299A URA3 2μ (pTH19) | This study |
| pTH160 | PADH1-GPA2Δ31-84 URA3 2μ (pTH19) | This study |
| pTH161 | PADH1-GPA2Δ16-84 URA3 2μ (pTH19) | This study |
| pTH163 | PADH1-MLS-GFP-GPB2 URA3 2μ (pTH19) | This study |
| pTH164 | PADH1-MLS-GFP-GPB1 URA3 2μ (pTH19) | This study |
| pTH166 | PADH1-NLS-GFP-GPB2 URA3 2μ (pTH19) | This study |
| pTH167 | PADH1-NLS-GFP-GPB1 URA3 2μ (pTH19) | This study |
| pTH168 | PADH1-GPA2Δ46-100 G299A URA3 2μ (pTH19) | This study |
| pTH169 | PADH1-GPA11-10-GPA2Δ1-14 URA3 2μ (pTH19) | This study |
| pTH170 | PADH1-GFP-GPR1C TRP1 2μ (pTH172) | This study |
| pTH171 | PADH1LEU2 2μ | This study |
| pTH172 | PADH1TRP1 2μ | This study |
| pTH173 | PADH1LYS5 2μ | This study |
| pTH174 | PADH1-GPB1 LYS5 2μ (pTH173) | This study |
| pTH178 | PADH1-GPA2Δ46-449 URA3 2μ (pTH19) | This study |
| pTH191 | PADH1-GPA11-10-GPA2Δ1-44 URA3 2μ (pTH19) | This study |
| pTH192 | PADH1-GPA11-10-GPA2Δ1-44 G299A URA3 2μ (pTH19) | This study |