Skip to main content
. 2005 Oct;16(10):4623–4635. doi: 10.1091/mbc.E05-01-0033

Figure 4.

Figure 4.

Establishment of normal human cells stably overexpressing each human SIRT. (A) NHF cells with the retroviral vector driving each SIRT (+SIRT1–7) at 13 PDs and 45 PDs were examined for mRNA expression of the respective SIRT gene by the real-time quantitative RT-PCR assays. NHF with the empty vector (Control) was also examined for the endogenous expression of each SIRT gene. The data are expressed as relative values to the endogenous expression levels in control cells (defined as 1 for each SIRT gene). (B) Western blot showed the overexpression of SIRT1, SIRT2, SIRT6, or SIRT7 protein in the respective retroviral vector-transduced NHF. The antibodies specific to each SIRT protein were used. (C) Western blot showed the overexpression of HA-SIRT1, HA-SIRT2, HA-SIRT6, or HA-SIRT7 protein in the respective retroviral vector-transduced NHF. Anti-HA antibody was used. (D) PrEC cells with the retroviral vector driving each SIRT (+SIRT1–7) or the empty vector (Control) were examined at 11 PDs as in A.