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Endogenous SIRT expression during replicative aging in normal human fibroblasts. (A) The real-time RT-PCR assays quantitated mRNA expression of each SIRT gene in NHF (at 23, 33, and 41 PDs) and WI-38 (at 34 PDs, 42 PDs, and 54 PDs). (B) The same set of cells as in (A) was examined in Western blot for SIRT1, SIRT6, and SIRT7 protein expression. β-actin was a loading control.
