Figure 3.

Osmotic defects of alr-1 mutant worms. Worms were assayed by placing individuals within an encircling barrier of 4 M NaCl (diameter of 1.6 mm for adults, 0.8 mm for L2 larva) as described previously (Culotti and Russell, 1978). Avoidance was scored as the inability of worms to cross the barrier within 15 min. Controls were assayed in the absence of 4 M NaCl. Strains used were N2 (wild type), osm-3(p802), alr-1(ok545), and alr-1(ok545) containing an integrated transgene expressing the wild-type alr-1 cDNA via the itr-1pB promoter (Gower et al., 2001). At least 200 individuals were assayed for each strain and stage. Asterisks indicate a significant difference (p < 0.005, via chi-square test) from that of the alr-1(ok545) strain. Error bars represent 95% confidence intervals.