Figure 5.

ArfGAP1 overexpression reduces membrane association of AP-1 at the trans-Golgi network and on endosomes. COS-1 cells were transfected with myc-tagged full-size ArfGAP1 (A and B) or catalytic domain ArfGAP1(6–136) (C and D) and stained for AP-1 (anti-γ adaptin; A and C) and for the myc-epitope to visualize transfected cells (B and D). Transfected cells are indicated by asterisks in A and C. AP-1 localization in the perinuclear Golgi area and on endosomes in the cell periphery is strongly reduced in cells expressing full-size ArfGAP1, but not in cells expressing the catalytic domain only.