Figure 2.

Pharmacological inhibition of γ-secretase impairs calcium signaling. (a) Typical BK-evoked calcium signals in N2aWt11 cells treated with various concentrations of MW-III-36C. (b) Effects of a representative γ-secretase inhibitor on both BK-evoked calcium signal amplitude (open circles) and [Aβ_1–40] in the conditioned medium (closed circles) after incubation in various concentrations of compound for 3–4 h. Calcium data are mean of quadruplicate determinations. ELISA data are mean of triplicate determinations. (c) The calcium deficits produced by γ-secretase inhibitors are reversible (see text for details). Data are mean ± SE of 6 experiments per condition. (d) Time course of effectiveness of IL on calcium signal amplitude (open circles) and [Aβ_1–40] in the conditioned medium (closed circles). Calcium data are mean ± SE of 4 experiments per condition; ELISA data are mean of triplicate determinations *, P < 0.01.