Figure 5.

Cells with impaired AICD production exhibit reduced ER calcium levels. To quantify ER calcium content, calcium stores were depleted with 1 μM thapsigargin in calcium-free medium. (a) Representative traces from control and PS1^−/−/PS2^−/− cells. (b–d) Amplitude of thapsigargin-evoked calcium transients in (b) PS1^−/−/PS2^−/− cells, (c) APP^−/− cells, and (d) in cells treated with various γ-secretase inhibitors (or PIA, an inactive control). Data are mean + SE of 4–6 experiments per condition. *, P < 0.01.