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. 2005 Oct;16(10):5013–5025. doi: 10.1091/mbc.E05-01-0065

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Copyright © 2005, The American Society for Cell Biology

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Figure 7. — Effect of caffeine on the phosphorylation of H2A.X. Top, staining of EBS (ATM^-/- cells) after 4-h incubation with the indicated concentration of caffeine. Bottom, corresponding image sets from the ATM restored YZ5 cell line. The arrows indicate cells that are in mitosis. The insets on the bottom of each panel show corresponding DAPI images for each field of cells shown. The inset at the top right corner of the 3 mM caffeine-treated EBS panel is a 1.5× magnification view of a contrast-enhanced image of the prometaphase cell highlighted with the arrow. The purpose is to illustrate the presence of staining along the chromosome arms of the mitotic chromosomes.