Table 2.
Quantitative analysis of normalized γ-H2AX signal intensity in mitotic and interphase cells
| Interphase population
|
Mitotic population
|
||||
|---|---|---|---|---|---|
| Cell linea | Mean ± SEMb | nc | Mean ± SEM | n | p Valued |
| HeLa | 0.120 ± 0.006 | 226 | 0.397 ± 0.040 | 36 | <0.0001 |
| M059K | 0.130 ± 0.006 | 483 | 0.721 ± 0.100 | 19 | <0.0001 |
| M059J | 0.185 ± 0.006 | 400 | 0.644 ± 0.053 | 27 | <0.0001 |
| GM38 | 0.085 ± 0.005 | 182 | 0.385 ± 0.069 | 20 | <0.0001 |
| AT2BE | 0.308 ± 0.050 | 141 | 0.340 ± 0.066 | 13 | 0.848 |
| AT5BI | 0.648 ± 0.012 | 305 | 0.698 ± 0.054 | 26 | 0.257 |
| EBS | 0.254 ± 0.050 | 392 | 0.278 ± 0.048 | 27 | 0.292 |
| YZ5 | 0.205 ± 0.013 | 457 | 0.654 ± 0.123 | 31 | <0.0001 |
M059J are DNA-PKcs deficient, AT2BE,AT5BI and EBS are ATM defective, and YZ5 are ATM corrected.
Mean Norm-γ-H2AX signal intensity ± SEM.
n, number.
A p value <0.05 is considered to be statistically significant.