Table 3.
Transcriptome data quality control.
| S: No | Feature | Value |
|---|---|---|
| Data filtering statistics | ||
| 1. | Raw data | 3,83,85,854 |
| 2. | Clean data (%) | 3,83,82,184 (99.99%) |
| 3. | Adapter (%) | 3,670 (0.01%) |
| 4. | Low quality (%) | 0 (0.00%) |
| 5. | Poly A (%) | 0 (0.00%) |
| 6. | N (%) | 0 (0.00%) |
| Base information statistics | ||
| 7. | Raw data (bp) | 5.75 Gb |
| 8. | BF-Q20(%) | 5.69 Gb (98.92%) |
| 9. | BF-Q30(%) | 5.54 Gb (96.22%) |
| 10. | BF-N (%) | 76,798 bp (0.00%) |
| 11. | BF- GC (%) | 3.39 Gb |
| 12. | Clean data (bp) | 5.75 Gb |
| 13. | AF-Q20 (%) | 5.68 Gb |
| 14. | AF-Q30 (%) | 5.53 Gb |
| 15. | AF-N (%) | 76,665 bp (0.00%) |
| 16. | AF-GC (%) | 3.38 Gb (58.91%) |
Low quality data has been filtered by removing reads containing adapters, reads with greater than 10% N content, A- base reads and low-quality reads. After filtering, data represents clean reads with Q20% - 5.68 Gb, Q30% - 5.53 Gb, N% - 76,665 bp and GC% - 3.38 Gb.