Table 4.

Statistical information of genome assembly, evaluation and prediction of T. sanguinea ZHSJ.

S: No	Feature	Value
Genome assembly
1.	Total contig number	135
2.	Total bases in contig	41324262 bp
3.	Large contig length	4110638 bp
4.	Contig N50	2813802 bp
5.	Contig N90	69383 bp
6.	G + C	52.08%
Genome assembly evaluation
BUSCO
7.	Complete	94.3%
8.	Complete duplicated	0.4%
9.	Fragmented	2.0%
10.	Missing	3.7%
CEGMA
11.	Complete	94.35%
12.	Partial	95.97%
13.	Total Orthologs number	247
14.	Average Orthologs number	1.06
Genetic prediction
Coding gene prediction
15.	Number of coding genes	9049
16.	Total gene length	23070809 bp
17.	Gene average length	2549.54 bp
18.	Gene density	0.22 kb
19.	GC content in gene region	57.39%
20.	Percentage of genes in genome	55.83%
21.	Intergenetic region length	18253453 bp
22.	GC content in intergenetic region	54.93%
23.	Genome percentage in intergenetic region	44.17%
24.	Transposable elements	209
rRNA prediction
25.	Total rRNAs	1035
26.	5S rRNA	259
27.	5.8S rRNA	258
28.	18S rRNA	256
29.	28S rRNA	262
Transcriptome assembly
30.	Number of genes	11861
31.	Gene coverage	96.37%
32.	GC (%)	57.9754%
33.	N50 number	2865
34.	N50 length	2316
35.	Maximum length	15161
36.	Minimum length	201
37.	Average length	1715
38.	Total assembled bases	2,03,45,744 bp
Denovo prediction
39.	Number of coding genes	12268
40.	Total gene length	20258617 bp
41.	Gene average length	1651.34 bp
42.	Gene density	3.25 kb
43.	GC content in gene region	56.91%
44.	Percentage of genes in genome	50.78%
45.	Intergenetic region length	19636407 bp
46.	GC content in intergenetic region	55.43%
47.	Genome percentage in intergenetic region	44.17%
48.	Transposable elements	209

The genome sequence and transcriptome sequence after quality control were subjected to assembly and genetic prediction.