Figure 1.

Wild-type and mutant Mcm2 and Mcm3 nucleocytoplasmic transport signals. (A) Key amino acid sequences of wild-type and mutant Mcm2 NLS and Mcm3 NLS-NES transport module. Putative NLSs and leucines in leucine-rich motif are in bold. Consensus CDK phosphorylation sites are underlined with putative phosphoacceptor residues in gray. Amino acids not spelled out are indicated in parentheses. Amino acid substitutions in the mutant Mcm proteins used in this study are indicated below the wild-type sequences. (B) A schematic of the MCM transport module containing the Mcm2 NLS and the Mcm3 NLS-NES. Below the schematic are the WT and corresponding mutant alleles of this construct that are discussed in the text.