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. 2005 Oct;16(10):5026–5039. doi: 10.1091/mbc.E05-05-0412

Figure 6.

Figure 6.

The Mcm3 NES is required for the timely nuclear export of Mcm3 and Mcm7. (A) Mutation of the Mcm3 NES delays the nuclear export of GFP-Mcm3. YJL2162 (GFP-MCM3) and YJL2741 (GFP-mcm3-nes) cells were arrested in G1 phase with alpha factor and at time 0 synchronously released into a G2/M phase arrest with nocodazole. At the indicated times, samples were taken for flow cytometry and fluorescence microscopy. (Left) Representative images from 0, 60, and 100 min. (Right) Flow cytometry profiles. (B) Mutation of the Mcm3 NES delays the nuclear export of Mcm7-GFP. YJL1979 (MCM3 MCM7-GFP) and YJL5439 (mcm3-nes MCM7-GFP) were treated and analyzed as described in C, except microscopic images from the 0-, 50-, and 70-min time points are shown.