Table 5.

Summary of the predicted gene structures using three methods.

Method	Software/Species	Number	Average length (bp)				Average exon per gene
			gene	CDS	exon	intron
De novo	Augustus	24,459	14,516.87	1,741.71	159.82	1,290.65	10.9
	Glimmer	39,727	14,042.91	1,020.96	161.29	2,443.21	6.33
Homolog	E. moara	50,987	23,273.19	1,658.37	180.14	2,634.1	9.21
	L. japonicus	51,010	19,380.37	1,656.83	179.59	2,154.73	9.23
	E. fuscoguttatus	52,748	23,530.9	1,656.82	180.53	2,674.84	9.18
	P. flavescens	53,847	24,587.81	1,638.19	181.46	2,858.73	9.03
	S. umbrosus	53,366	24,022.61	1,673.48	185.4	2,784.43	9.03
RNA-seq	PASA	21,217	16,814.96	3,699.28	302.27	1,167.04	12.24
Integrated	EVM	25,093	13,819.54	1,721.49	168.93	1,316.4	10.19