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. 2025 Aug 21;12:1457. doi: 10.1038/s41597-025-05735-w

Table 7.

Assessment metrics of the genome assembly and annotation.

Type Evaluation Methods Results
Genome accuracy and completeness Mapping short reads rate 99.46%
Mapping HiFi reads rate 99.99%
Mapping ONT reads rate 98.43%
QV Short reads 40.59
HiFi reads 57.80
CRAQ R-AQI 98.42%
S-AQI 99.45%
BUSCO 100%
Annotation quality Complete BUSCOs 98.8% (3,599)
Complete and single-copy BUSCOs (S) 98.2% (3,576)
Complete and duplicated BUSCOs (D) 0.6% (23)
Fragmented BUSCOs (F) 0 (0)
Missing BUSCOs (M) 1.2% (41)
RNA-seq coverage ratio of the exonic regions 94.71%