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Inhibition of F-actin depolymerization does not affect formation of VACs and endocytosis of TJ proteins. Confluent T84 monolayers were preincubated for 37 h with IFN-γ followed by incubation with either F-actin stabilizing drug jasplakinolide (1 μM) or vehicle for additional 1 h. Note that F-actin stabilization failed to inhibit either formation of VACs (arrows) or internalization of occludin (arrowheads). Scale bar, 10 μm.
