Figure 7.

Myosin II activity mediates formation of VACs and endocytosis of TJ proteins in IFN-γ-treated cells.T84 cells were preincubated with IFN-γ for 36 h followed by additional 4-h incubation with either the selective nonmuscle myosin II inhibitor blebbistatin (50 μM) or vehicle. Localization of occludin and F-actin was determined by immunofluorescence labeling/confocal microscopy. Note that inhibition of myosin II completely blocks formation of VACs and internalization of occludin in IFN-γ-treated cells. Scale bar, 10 μm.