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. 2025 Aug 21;16:7689. doi: 10.1038/s41467-025-62673-2

Fig. 1. Biobank establishment of small round cell sarcoma cancer tumoroids.

Fig. 1

A Flow diagram with an overview of the study design. Tumoroids were established from patient tumor and PDX material and subsequently characterized using whole genome sequencing, RNA sequencing and histology. Drug screens were performed on a subset of ES, CDS and BRS. Lastly, primary tumors and matching tumoroid lines were viably frozen and stored in liquid nitrogen. Created in BioRender. Ringnalda, F. (2025) https://BioRender.com/ovfd63d. B Donut chart (left) representing the different fusions and the number of patients per fusion that were included in the ES, CDS and BRS biobank. Bar chart (right) of the total number of established tumoroid lines from each fusion, subdivided in tumoroids derived from patient tumors and those derived from PDX material. C Representative brightfield microscopy images of EWSR1::FLI1, EWSR1::ERG, EWSR1::FEV, BCOR::CCNB3, KMT2D::BCOR and CIC::DUX4 tumoroids (n = 3, frames of individual area of interest). Scale bar: 200 µm, zoom-in 100 µm. ES Ewing sarcoma, CDS CIC::DUX4 sarcoma, BRS BCOR-rearranged sarcoma. Source data are provided as a Source Data file.