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. Author manuscript; available in PMC: 2005 Sep 29.
Published in final edited form as: Neuroreport. 2005 Aug 1;16(11):1203–1207. doi: 10.1097/00001756-200508010-00014

Fig.2.

Fig.2

Effects of dehydroepiandrosterone-sulfate (DHEA-S) on DNA damage and death of retinal pigment epithelial (RPE) cells induced by H2O2. (a) Primary retinal pigment epithelial (RPE) cells were preincubated with DHEA-S (1–100 μM) and then exposed to 50 μM of H2O2 with or without N-[2-(3,4-dichlorophenyl)ethyl]-N-methyl-2-(dimethylamino) ethylamine (BD1047) (the concentration used was 100 μM for both BD1047 and DHEA-S). DNA strand breaks were determined after 20 min of H2O2 exposure. (b) ARPE-19 cells were preincubated with DHEA-S (1–100 μM) with or without BD1047 (100 μM) and then exposed to 400 μM H2O2. Cell viability was measured after 4 h of H2O2 exposure. Each bar shows the mean ± SD of 4–6 experiments. *p < 0.05, **p < 0.01 versus control (CTR; H2O2 alone); ***p < 0.05 versus DHEA-S (100 μM).