Fig. 2.
Levels of Pol IV are increased in a recG mutant strain but are not affected by ruv mutations. Western blot showing levels of Pol IV in (from left to right) wild type = FC40; recG = FC465; Δ (ruvAC) = FC836; recG Δ (ruvAC) = FC897; ΔdinB = FC1240. Samples consisting of 10 μg of total protein were loaded in each lane of a precast SDS-PAGE gel (Bio-Rad Laboratories). The intensities of the bands for each strain relative to the wild-type strain are given below each lane; the intensity of only the upper band relative to that of the wild-type strain is in parenthesis.