Fig. 4.
An rpoS mutation reduces the cellular levels of Pol IV protein. A. Western blot showing levels of Pol IV in (from left to right) ΔdinB = FC1240; recG rpoS = PFG36; recG = FC526; wild type = FC40. B. A different Western blot showing levels of Pol IV in (from left to right) wild type = FC40; rpoS = PFG72. Samples consisting of 40 μg of total protein were loaded in each lane of each blot; the blot in B was developed long enough to enhance the band from the rpoS mutant strain. The intensities of the bands for each strain relative to the wild type strain are given below each lane; the intensity of only the upper band relative to that of the wild type strain is in parenthesis.